A method for generating nonpathogenic, infectious pancreatic necrosis virus (ipnv) from synthetic rna transcripts
CA 2324478 C · Owner: Yao, Kun · Inventors: Vakharia, Vikram N.; Yao, Kun
Overview
Its claims, description and drawings are in the Canadian Patents Database.
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A system for the generation of live, nonpathogenic infectious pancreatic necrosis virus (IPNV), a segmented double-stranded (ds)RNA virus of the Birnavirdae family, using synthetic transcripts derived from cloned DNA has been developed. Independent full-length cDNA clones were constructed which contained the coding and non-coding regions of RNA segments A and B of IPNV respectively. Segment A was modified to prevent the expression of NS protein. Synthetic RNAs of both segments were produced by in vitro transcription of linearized plasmids with T7 RNA polymerase. Transfection of CHSE cells with combined plus-sense transcripts of both segments generated infectious virus. The …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
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- Filed March 31, 1999 and granted August 23, 2011. A Canadian patent filed from October 1, 1989 lasts 20 years from filing, so its term ended March 31, 2019.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by March 31, 2021.
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Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.