Multi-zone polymerase/ligase chain reaction

Abstract From the Canadian patent

A process of amplifying a nucleic acid sequence by a procedure involving a nucleic acid charin reaction (e.g. a polymerase chain reaction or a ligase chain reaction). The process involves repeated cycles of steps including a nucleic acid denaturing step and a nucleic acid synthesis step the synthesis , step being carried out under the action of an enzyme (a nucleic acid polymerase or ligase). The denaturing step and the synthesis step are carried out in different denaturing and synthesis reaction zones respectively, and, during the repeated cycles the enzyme is maintained in isolation from the denaturing reaction zone, and conditions or reagents required for the denaturing step are …