Recombinant alphavirus-based vectors with reduced inhibition of cellular macromolecular synthesis

Abstract From the Canadian patent

Isolated nucleic acid molecules are disclosed comprising an alphavirus , nonstructural protein gene which when operably incorporated into a , recombinant alphavirus particle eukaryotic layered vector initiation system or RNA vector replicon, has a reduced level of vector-specific RNA synthesis as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter as compared to a wild-, type recombinant alphavirus particle. Also disclosed are RNA vector replicons alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.