Methods for covalent immobilisation of biomolecules to a carrier by means of a his-tag
CA 2293777 A1 · Owner: Van Den Broeck, Dirk · Inventors: Bosman, Alfons; Van De Voorde, Andre; Van Wijnendaele, Frans
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
The present invention relates to methods for covalent immobilisation of biomolecules to carriers and membranes wherein the presence of a His-tag is , exploited and wherein the amino acid residues that comprise said His-tag are directly involved in the covalent bond. The present invention also provides several strategies that further augment the probability of covalent immobilisation through said His-tags, such as improving the presentation of said His-tag, choosing the appropriate reaction conditions such as pH temperature, concentration of reagent and kinetics increasing contact between , His-tag and reactive groups of said carrier or membrane by for instance the , use of IDA or …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed June 25, 1998. No grant is recorded for it in Canada; had it been granted, its 20-year term would have ended June 25, 2018.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by June 25, 2020.
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Timeline
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Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.