Assay of peroxidase activity

Abstract From the Canadian patent

Chemiluminescent detection of molecules of synthetic or natural origin such as proteins and nucleic acids (DNA and RNA) as well as other biologic molecules, is increasingly replacing radioactive detection as the method of choice where sensitivity is critical. In such assays luminescence is customarily achieved , by the oxidation of a luminol or isoluminol substrate in the presence of an oxidizing agent such as hydrogen peroxide or hydrogen peroxide source such as , perborate, and a peroxidase catalyst such as horseradish peroxidase. To obtain useful levels of luminescence (e.g. detectable levels) by customary techniques, a luminescent enhancer is also present during oxidation. It has …