Genetically modified cells and their use in the prophylaxis or therapy of disorders

Abstract From the Canadian patent

The invention relates to cells for use in gene therapy obtainable by, a) isolation of cells from the blood or cell-containing fluids of the body; b) culturing of the cells obtained in step a) in a cell culture medium comprising gangliosides, phospholipids glycolipids and/or growth, factors;, c) alternatively immortalization of the cells obtained in step a) or b) by transformation with an oncogene, activation of an oncogene or inactivation of a suppressor gene; and d) alternatively transfection of the cells obtained in step a) and b) or in step c) with a nucleic acid construct for gene therapy comprising an, effector gene which can be activated target cell-specifically cell, …