Methods, apparatus and computer program products for determining quantities of nucleic acid sequences in samples

Abstract From the Canadian patent

Methods for determining quantities of nucleic acid sequences in samples include the steps of amplifying a plurality of known quantities of a nucleic acid sequence in respective calibration samples and an unknown quantity of a nucleic acid sequence in a test sample in parallel, during a, time interval. These samples may be amplified using an isothermal amplification method such as Strand Displacement Amplification (SDA) or a, thermal cycling amplification method such as Polymerase Chain Reaction (PCR), for example. Indicia of the quantities of a nucleic acid sequence being amplified in the calibration and test samples are then measured using conventional techniques, at measurement points in …