Dual chamber disposable reaction vessel for amplification reactions, reaction processing station therefor, and methods of use

Abstract From the Canadian patent

A reaction vessel for a nucleic acid amplification reaction has a first chamber containing an amplification reagent mix a second chamber containing an amplification enzyme, and a fluid channel or chamber connecting the first and second chambers together. A fluid sample is introduced into the first chamber. After a denaturation and primer annealing process has occurred in the first chamber the fluid channel is opened to, allow the solution of the reagent and fluid sample to flow into the second chamber. The second chamber is maintained at an optimal temperature for the amplification reaction. A station is described for processing test strips incorporating the reaction vessels. The station …