Method for isolating xylanase gene sequences from soil dna, compositions useful in such method and compositions obtained thereby

Abstract From the Canadian patent

Xylanase DNA is recovered from soil by PCR amplification using degenerate primers. Because of the complexity of the soil samples it is likely that the , recovered product will include more than one species of polynucleotide. These recovered copies may be cloned into a host organism to produce additional copies of each individual species prior to characterization by sequencing. Recovered DNA which is found to vary from known xylanases can be used in several ways to facilitate production of novel xylanases for industrial application. First, the recovered DNA, or probes corresponding to portions thereof, can be used as a probe to screen DNA libraries and recover intact xylanase genes …