Improvements in the in situ pcr
CA 2218875 C · Owner: The Research Foundation Of State University Of New York · Inventors: Nuovo, Gerard J.; Bloch, Will
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
Improvements to the in situ polymerase chain reaction (PCR) a process, of in vitro enzymatic amplification of specific nucleic acid sequences within the cells where they originate, can be achieved by changing the way that the enzymatic reaction is started. Reaction initiation is delayed until the start ofPCR thermal cycling either by withholding a subset of PCR reagents from the cellular preparation until the preparation has been heated to 50° to 80° C immediately before thermal cycling is begun or by adding to the PCR reagents a, single-stranded DNA binding protein which blocks reaction at temperatures below about 50°C. If the in situ PCR is performed on cellular preparations already …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed July 20, 1992 and granted November 7, 2000. A Canadian patent filed from October 1, 1989 lasts 20 years from filing, so its term ended July 20, 2012.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by July 20, 2014.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
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Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.