Purified stat proteins and methods of purifying thereof
CA 2218456 A1 · Owner: Darnell, James E. Jr. · Inventors: Darnell, James E. Jr.; Vinkemeier, Uwe
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
The present invention describes methods of producing milligram quantities of three forms of puriried Statl protein from recombinant DNA constructs. In addition the Stat proteins, may be isolated in their phosphorylated or nonphosphorylated forms (Tyr 701). The proteins can be produced in baculovirus infected insect cells or E. coli. A compact, domain in the amino terminus of Stat .alpha. was isolated and found to enhance DNA binding due to its abiliry to interact with a neighboring Stat protein. A relatively protease-resistant recombinant truncated form of the Stat protein was isolated in 40-50 mg quantities. Purification of the Stat proteins were performed after modifying specific cysteine …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed October 15, 1997. No grant is recorded for it in Canada; had it been granted, its 20-year term would have ended October 15, 2017.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by October 15, 2019.
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Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.