Generation of human cytotoxic t-cells specific for carcinoma self-associated antigens and uses thereof

Abstract From the Canadian patent

We have discovered that by using a recombinant DNA viral vector preferably a , pox virus vector having at least one insertion site containing a DNA segment encoding the carcinoma self-associated antigen or a cytotoxic T-cell , eliciting epitope thereof operablylinked to a promoter capable of expression in the host, human cytotoxic T-cells specific for the carcinoma selfassociated antigens can be produced. The method preferably comprises introducing a sufficient amount of the recombinant pox virus vector into a host to stimulate production of cytotoxic T-cells and contacting the host , with additional antigen at periodic intervals thereafter. The additional antigen may be added by using …