Method for molecular cloning and polynucleotide synthesis using vaccinia dna topoisomerase
CA 2208062 C · Owner: Shuman, Stewart · Inventors: Shuman, Stewart
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
This invention provides a modified vaccinia topoisomerase enzyme containing an affinity tag which is capable of facilitating purification of protein-DNA complexes away from unbound DNA. This invention further provides a modified sequence specific topoisomerase enzyme. This invention provides a method of ligating duplex DNAs, a method of molecular cloning of DNA a method of , synthesizing polynucleotides and a method of gene targeting. Lastly this , invention provides a recombinant DNA molecule composed of segments of DNA which have been joined ex vivo by the use of a sequence specific topoisomerase and which has the capacity to transform a suitable host cell comprising a DNA sequence …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed December 12, 1995 and granted September 27, 2011. A Canadian patent filed from October 1, 1989 lasts 20 years from filing, so its term ended December 12, 2015.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by December 12, 2017.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
Timeline From CIPO dates
Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.