Expression and export technology of proteins as immunofusins

Abstract From the Canadian patent

Disclosed are DNAs produced by recombinant techniques for inducing the expression and subsequent secretion of a target protein. The DNAs encode in , their 5' to 3' direction, a secretion cassette including a signal sequence , and an immunoglobulin Fc region and a target protein. The DNAs can be transfected into a host cell for the expression production and subsequent , secretion of the target protein as a fusion protein. The secreted protein can be collected from the extracellular space and further purified as desired. , The secreted fusion protein additionally can be proteolytically cleaved to release the target protein from the secretion cassette.