Hybridization and footprinting methods to characterize the interactions of 16s rrna analogues for identification of novel antibiotics

Abstract From the Canadian patent

The oligoribonucleotide analogs of the invention as exemplified in the , figure, are relatively small three-dimensional structures derived from larger parental RNA molecules. The analogs include a first nucleic acid structure including one or more nucleotide sequences that are derived from a region of parental RNA, wherein in its native state the region binds to a ligand, e.g. an aminoglycoside with a parental RNA ligand binding pattern and a second , nucleic acid structure including one or more nucleotide sequences combined with the first nucleic acid structure to form the analog and provide the analog with a conformation that binds the ligand with a ligand binding pattern that is …