Acetic anhydride activation for c-terminal protein sequencing
CA 2197304 C · Owner: Pe Corporation (Ny) · Inventors: Boyd, Victoria L.; Loudon, G. Marc; Bozzini, Merilisa
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
A method is provided for C-terminal sequencing of a protein or peptide. An important feature of the method is the formation of an oxazolone moiety at the C-terminus of a protein or peptide by treatment with acetic anhydride under basic conditions followed by conversion of the oxazolone to a thiohydantoin moiety by treatment with thiocyanate under acidic conditions. Yields of thiohydantoin are further enhanced by delivering thiocyanate as the conjugate acid of a sterically hindered alkylammonium cation.
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed August 29, 1995 and granted April 16, 2002. A Canadian patent filed from October 1, 1989 lasts 20 years from filing, so its term ended August 29, 2015.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by August 29, 2017.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
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Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.