Extraction of heterologous insoluble proteins from bacteria
CA 2192184 C · Owner: Zhu, Jingdong · Inventors: Zhu, Jingdong; Condon, Russell; Alroy, Yair
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
An insoluble mammalian protein is ex-, tracted from transformed bacteria expressing the mammalian protein while avoiding irreversible insolubilization of bacterial host proteins by homogenizing the fermentation broth, centrifuging the homogenized broth and removing the supernatant liquid from the inclusion body containing pellet. In another embodiment, the pH of the homogenized, broth is adjusted to 2.0 prior to centrifugation. The acidified broth is then centrifuged and the pellet is resuspended in buffer ho-, mogenized again, and the inclusion body is isolated by centrifugation.
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed June 19, 1995 and granted April 18, 2000. A Canadian patent filed from October 1, 1989 lasts 20 years from filing, so its term ended June 19, 2015.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by June 19, 2017.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
Timeline From CIPO dates
Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.