A purified mixture of collagenases and two other proteases obtained from clostridium histolyticum

Abstract From the Canadian patent

A purified enzyme mixture useful for isolating cells or cell clusters from tissue is disclosed. The mixture includes at least two collagenase enzymes, at least two other proteases and additional non-protease components. The mixture is purified by removing at least some of the non-protease components. The purified mixture may then be used to isolate cells or cell clusters from tissue. Also disclosed are the essential components of the purified enzyme mixture as well as preferred ranges and ratios of these essential components for isolating cells or cells clusters from tissue. Finally a dissociation system is disclosed that can be used with the purified enzyme mixture to dissociate tissue and …