Preparation of virally inactivated intravenously injectable immune serum globulin

Abstract From the Canadian patent

Method of reducing the anticomplement activity (ACA) resulting from viral inactivation treatment of a solution of antibodies the method comprising , contacting the solution with a trialkylphosphate, such as tri-n-butyl phosphate and a , detergent, such as , sodium cholate under conditions sufficient to reduce substantially the virus activity, and then , incubating the solution under controlled conditions of time pH, temperature, and ionic , strength such that the anticomplement activity is reduced to an acceptable level. In a preferred , embodiment, the ACA is reduced to less than 60 CH50 units/mL the incubation , is for at least , about ten days at a pH from 3.5 to 5.0 the temperature …