Chromosomal expression of heterologous genes in bacterial cells
CA 2179656 C · Owner: Olson, Pamela S. · Inventors: Mascarenhas, Desmond; Olson, Pamela S.
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
The present invention provides compositions and methods for inserting a copy of a heterologous gene into the chromosome of a host cell such as a E. coli through the use of a chromosomal transfer DNA a circular, non-self replicating DNA carrying a site-specific recombination site such as lambda att P. The host cell chromosome contains a second site-specific recombination site. When the chromosomal transfer DNA is introduced into the host cell, expression of an integration enzyme such as integrase causes the integration of the chromosomal transfer DNA into the host cell chromosome at the second recombination site.
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed December 21, 1994 and granted October 9, 2001. A Canadian patent filed from October 1, 1989 lasts 20 years from filing, so its term ended December 21, 2014.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by December 21, 2016.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
Timeline From CIPO dates
Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.