Immobilized mismatch binding protein for detection or purification of mutations or polymorphisms

Abstract From the Canadian patent

A method for detecting mutations, such as a single base change or an addition or deletion of about one to four base pairs is based, on the use of an immobilized DNA mismatch-binding protein such as MutS, which binds to a nucleic acid hybrid having a single base mismatch or unpaired base or bases, thereby allowing the detection of mutations involving as little as one base change in a nucleotide sequence. Such a method is useful diagnosing a variety of important disease states or susceptibilities detecting the presence of a mutated oncogene and for isolating or removing by affinity chromatography duplex DNA molecules containing mismatches such as error-containing molecules in PCR-amplified …