Method of increasing the catalytic activity of a glycanase having two or more catalytic domains

Abstract From the Canadian patent

A method for increasing the catalytic activity of a glycanase having two or more independent catalytic domains by isolating the catalytic domains from the intact enzyme is described. If the catalytic domains in the intact enzyme are linked by a proline/hydroxyamino acid-rich linker sequence the catalytic domains can be isolated and activated by cleavage within or adjacent to the linker sequence with a protease. The catalytic domains can also be isolated, whether or not they are linked by a hydroxyamino acid-rich linker sequence, by expressing the DNA sequences encoding each domain in separate transformed microbial hosts. An isolated and purified low-activity xylanase denoted XynC, and which …