Process for producing trans-4-hydroxy-l-proline

Abstract From the Canadian patent

Provided is a process for industrially producing trans-4-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process L-, proline is converted into trans-4-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Dactylosporangium or Amycolatopsis and which catalyzes the hydroxylation of Lproline into trans-4-hydroxy-L-proline a divalent iron ion, and 2-ketoglutaric acid in an aqueous medium, and the, produced trans-4-hydroxy-L-proline is collected from the aqueous medium. Also provided is a novel enzyme L-proline-4hydroxylase, which is useful for the process.