Site-directed mutagenesis of dna
CA 2126438 C · Owner: Nickoloff, Jac A. · Inventors: Deng, Win P.; Nickoloff, Jac A.; Ray, Frank A.
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
The invention pertains to a, method of site-specific mutagenesis of DNA. In this method, a parental DNA, containing a non-essential unique, restriction site is used to generate progeny DNA containing the desired, mutation beta lacking the restriction site. The non-mutant parental DNA and the mutant progeny DNA are treated with, any enzyme that cleaves at the restriction site to cleave the parental DNA; leaving the mutant DNA, uncleaved. Mutant progeny DNA is selected by transforming cells with the enzyme-treated DNA under conditions, in which the cells are transformed with uncleaved DNA at a higher efficiency, than with- cleaved DNA so that the majority of transformants carry the, mutant …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed December 17, 1992 and granted December 2, 2003. A Canadian patent filed from October 1, 1989 lasts 20 years from filing, so its term ended December 17, 2012.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by December 17, 2014.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
Timeline From CIPO dates
Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.