A method for detecting nitric oxide, nitrosonium equivalents, s-nitrosothiols and s-nitroso-proteins in biological systems

Abstract From the Canadian patent

Nitric oxide, nitrosoni-, nm, S-nitrosothiols and S-, nitroso-proteins can be de-, tested in biological samples using a method which in-, volves injection of samples into a photolysis cell, prior to, detection of chemiluminescence generated by the reac-, tion between nitric oxide and see Formula I ozone. To detect S-nitrosoth-, iols and S-nitroso-proteins separate aliquots of the same, samples are subjected to pretreatment with mercurous, ion, and a protein-precipitating agent, respectively, and, the resulting nitric oxide signals are compared to those, generated by untreated samples. This method is useful, for monitoring the levels of nitric oxide bioactivity in, both normal physiological …