Peptide and protein fusions to thioredoxin and thioredoxin-like molecules

Abstract From the Canadian patent

This invention provides a fusion molecule comprising a DNA sequence encoding a thioredoxin-like protein fused to the, DNA sequence encoding a selected heterologous peptide or protein. The peptide or protein may be fused to the amino terminus of the thioredoxin-like molecule, the carboxyl terminus of the thioredoxinlike molecule, or within the thioredoxin-like molecule for example at the active-site loop of said molecule. Expression of this fusion molecule under the control of a regulatory sequence capable of directing its expression in a desired host cell produces high , levels of stable and soluble fusion protein. The fusion protein, located in the bacterial cytoplasm may be selectively …