Method of preparing specimen for classifying and counting leukocytes

Abstract From the Canadian patent

32-, A hematological specimen for classifying and counting leukocytes with a flow cytometer is prepared. A sample to be assayed is prepared by eliminating influences of erythrocytes from a hematological sample without changing leukocytes morphologically by adding a first aqueous solution of a low osmotic pressure comprising a buffer for adjusting the pH value within an acidic region and a second aqueous solution comprising an osmolarity compensating agent and a buffer for giving a pH value suitable for staining optionally further adding a salt, which dissociates into ions in aqueous solutions so as to control the electrical conductivity of the aqueous solution at a preferable level while …