Recovery of bt endotoxin protein from lysed cell mixtures
CA 2070649 A1 · Owner: English, Leigh H. · Inventors: English, Leigh H.
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
Purified endotoxin protein is recovered from aqueous suspensions containing lysed cells and Bacillus thuringiensis crystalline endotoxin protein in a multistep process. The aqueous suspension, e.g. concentrated fermentation culture, is first treated under strongly basic or acidic conditions to solubilize the crystal protein and the solution is then separated from residual solids. Endotoxin protein is precipitated from the separated aqueous solution, by adjusting its pH to the isoelectric point of the protein, and recovered by centrifugation or the like.
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed December 4, 1990. No grant is recorded for it in Canada; had it been granted, its 20-year term would have ended December 4, 2010.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by December 4, 2012.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
Timeline From CIPO dates
Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.