Method and reagent composition for subtyping lymphocytes in peripheral blood
CA 2070244 A1 · Owner: Kim, Young Ran · Inventors: Paseltiner, Lynn; Yeh, Chien-Kuo; Kim, Young Ran
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
A rapid method for detection of monoclonal antibody labeled cells using forward light scatter/absorption clinical flow cytometers is disclosed. Calf-intestinal alkaline phosphatase is conjugated to mouse monoclonal antibodies (anti-CD2 CD3, CD4, CD8, CD19) for direct immunoenzymatic labeling. Red cells are pre-conditioned for lysis during the first incubation period with the alkaline phosphatase conjugated monoclonal antibodies by ammonium-sulfate which is followed by an early fixation of white cells in the presence of dextrose. The combination of 5-bromo-4-chloro-3-indolyl phosphate and nitroblue-tetrazolium salt in diethanolamine buffer, containiny zinc glycinate at pH, 9.5?0.1, is used …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed June 2, 1992. No grant is recorded for it in Canada; had it been granted, its 20-year term would have ended June 2, 2012.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by June 2, 2014.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
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Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.