Glycosylated haemoglobin assay

Abstract From the Canadian patent

This invention provides a method of assessing glycosylated haemoglobin in a sample, said method comprising the steps of: (a) optionally haemolysing the sample to liberate any cell bound haemoglobin; (b) contacting the said sample or haemoglobin recovered from said sample according to step (c) below with signal-forming molecules comprising a conjugate of one or more dihydroxyboryl residues or salts thereof linked to a signal-forming label; , (c) separating glycosylated and non-glycosylated haemoglobin and any molecules bound thereto from the sample or from the , reaction mixture of step (b) above; and (d) assessing the said signal forming molecules which have bound to the separated …