Tmb formulation for soluble and precipitable hrp-elisa
CA 2045522 A1 · Owner: Woiszwillo, James E. · Inventors: Woiszwillo, James E.
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
Solvent systems for use in increasing the solubility of 3,3',5',5'-tetramethylbenzidine (TMB) and for making a permanent color record of the results of ELISAs using HRP have been developed. In the improved solvent system containing povidone, 1-ethenyl-2-pyrrolidinone polymers TMB can be, dissolved in a very small quantity of solvent up to a, concentration of 100 to 200 mM which can then be mixed, directly into an aqueous buffer without precipating the TMB. In the solvent system for making a permanent record alginic, acid (AA), methyl vinyl ether/maleic anhydride copolymer (MVE/MAC), dextran sulfate (DS) and/or carrageenan are added to the aqueous buffer solution, with or without povidone …
Read the whole patent
Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed November 2, 1990. No grant is recorded for it in Canada; had it been granted, its 20-year term would have ended November 2, 2010.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by November 2, 2012.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
Timeline From CIPO dates
Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.