Reverse sample genome probing of sulphate-reducing bacteria

Abstract From the Canadian patent

A reverse genome probe process for detecting the presence of sulfate-reducing bacteria consists of: (a) obtaining an individual culture of at least one "selected standard" (which selected standard may be either a single sulfate-reducing bacterium or a group of more than one sulfate-reducing bacteria having homologous, cross-hybridizing genomes); (b) affixing a nucleic acid preparation from each selected standard to a solid surface;, (c) preparing a "reverse" probe by labeling a nucleic acid preparation obtained from a sample taken from an oil field environment; (d) contacting the reverse probe with the solid surface under conditions which permit hybridization; and (e) detecting the presence …