Cephalosporin acetylhydrolase gene and protein encoded by said gene

Abstract From the Canadian patent

The invention relates to a cephalosporin acetylhydrolase gene, a recombinant DNA molecule prepared by introducing the gene into a vector used in an E. coli host-vector system E. coli cells transformed with the recombinant DNA molecule a, protein having the amino acid sequence encoded by the gene an, enzyme which is a multimer of the protein and a process for, preparing the protein or enzyme. The cephalosporin acetylhydrolase is an enzyme useful for converting cephalosporins, e.g. cephalosporin C and 7-ACA into, deacetylated ones, e.g. deacetylcephalosporin C and deacetyl 7-ACA, which are useful as intermediates in the preparation of a variety of derivatized cephalosporin antibiotics.