Method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
CA 2035010 C · Owner: Biotechnica International, Inc. · Inventors: Carrino, John J.; Backman, Keith C.; Bond, Sheila B.
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
The present invention involves methods of improving LCR and PCR amplification schemes by modifying at least one probe/primer end so that the probability of the probe/primer contributing to spurious signal development is greatly reduced. Only after specific hybridization of the modified probe/primer with true target are the, modified ends "corrected" in a target dependent fashion to allow participation of the probe/primer in the enzymatic assembly reaction. Specific modifications depend on whether the assembly is done by ligation (as in LCR) or by extension/elongation (as in PCR).
Read the whole patent
Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed January 25, 1991 and granted December 10, 1996. A Canadian patent filed from October 1, 1989 lasts 20 years from filing, so its term ended January 25, 2011.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by January 25, 2013.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
Timeline From CIPO dates
Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.