Reagent and method of using same for the automatic flow cytometry determination of at least one leukocyte sub-population from total blood

Abstract From the Canadian patent

The invention relates to a reagent and to a method of using same for automatically determining at least one leukocyte sub-population from a total blood sample. Thanks to this reagent, it is possible in a single step, to lyse the, erythrocytes through the action of saponin and SDS as well as to ensure the, differential staining of the leukocyte sub-populations at the same time preserving the, integrity and morphology of the cells through the combined action of various components including chlorazol black, an alcohol a surfactant and preserving agents. The reagent according to the invention can be applied to different types of automatic flow cytometry analyzers.