Nucleic acid detection method using unequal primer concentrations in polymerase chain reaction
CA 2024978 A1 · Owner: Wu, Annie Liu · Inventors: Wu, Annie Liu; Wu, Annie L.
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
Abstract of the Disclosure, The detection of nucleic acids can be achieved with certain advantages in polymerase chain reactions by using primers in unequal concentrations. In particular, the molar ratio of a pair of primers is at least 2:1. Amplification of the nucleic acid of interest is carried out using standard reagents, but denaturation of the complementary products prior to detection is not needed. Detection is then accomplished using an enzymelabeled reagent which will provide a colorimetric or fluorometric signal in the presence of the appropriate substrate.
Read the whole patent
Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed September 10, 1990. No grant is recorded for it in Canada; had it been granted, its 20-year term would have ended September 10, 2010.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by September 10, 2012.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
Timeline From CIPO dates
Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.