Enhancement of naphthalene dioxygenase activity during microbial indigo production
CA 2022285 C · Owner: Serdar, Cuneyt M. · Inventors: Serdar, Cuneyt M.; Murdock, Douglas C.; Ensley, Burt D. Jr.
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
This invention relates to a process for the enhancement of naphthalene dioxygenase activity in organisms that have been transformed with DNA encoding the expression of a multiple component naphthalene dioxygenase enzyme. Cells containing the naphthalene dioxygenase enzyme are capable of producing indigo when cultured in the presence of indole. The invention includes procedures to enhance naphthalene dioxygenase activity by transforming a host cell with a DNA sequence that encodes at least two ferredoxin polypeptides for each reductase polypeptide and iron-sulfur polypeptide by growing transformed organisms in the presence of iron, or by transforming a host cell with a DNA sequence that …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed July 30, 1990 and granted December 31, 2002. A Canadian patent filed from October 1, 1989 lasts 20 years from filing, so its term ended July 30, 2010.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by July 30, 2012.
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Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.