Large-scale fermenter inoculation with frozen cells
CA 2015583 C · Owner: Miles Inc. · Inventors: Trawinski, Jurgen; Schwartz, Barry D.
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
An improved method of inoculating large scale fermenters with mammalian cells is provided. Instead of taking one or more ampules of frozen cells through a series of scale up steps in tissue culture flasks and/or roller bottles the, cells are inoculated into a small fermenter in advance of their anticipated usage in fermenter inoculation. The cells are concentrated and dispensed into approximately 500 ml aliquots (or larger) and mixed with a cryopreservative solution. The aliquots are frozen in liquid nitrogen in specially designed freezer bags. The cells can thus be held indefinitely. When a fermenter run is to be initiated, a cell bag can simply be thawed and inoculated into the fermenter. …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed April 27, 1990 and granted June 25, 2002. A Canadian patent filed from October 1, 1989 lasts 20 years from filing, so its term ended April 27, 2010.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by April 27, 2012.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
Timeline From CIPO dates
Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.