Humanized immunoglobulins and their production and use

Abstract From the Canadian patent

Novel methods for designing humanized, immunoglobulins having one or more complementarity determining regions (CDR's) from a donor immunoglobulin and a framework region from a human immunoglobulin comprising first comparing the framework or variable region amino acid sequence of the donor immunoglobulin to corresponding sequences in a collection of human immunoglobulin chains and, selecting as the human immunoglobulin one of the more homologous sequences from the collection. Each humanized immunoglobulin chain may comprise about 3 or more amino acids from the donor immunoglobulin in addition to the CDR's usually at least one of which is immediately adjacent to a CDR in the donor …