Thrombolytic proteins, process for producing the same, and drugs containing the same as active ingredient

Abstract From the Canadian patent

Novel thrombolytic proteins having an amino acid sequence similar to that of natural human tissue plasminogen activator in which at least one asparagine-linked oligosaccharide chain is attached to a sequence in its epidermal growth factor domain; DNA molecules that code for the amino acid sequences of the novel thrombolytic proteins; and process for producing proteins which comprises introducing the DNA molecule into an expression vector capable of establishing itself in host cells that allow addition of asparagine-linked oligosaccharide chain transforming said host cells by said expression vector culturing the transformants thus obtained under conditions that allow expression of said DNA …