Expression induction method
CA 2004632 C · Owner: Gillies, Stephen D. · Inventors: Gillies, Stephen D.; Lo, Kin-Ming
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
Disclosed is a method of producing increased amounts of a protein of interest in a cell by induction. The method includes transfecting a cell with multiple copies of an expression vector each, copy of which includes an espressible gene encoding an enzymatically functional dihydrofolate reductase (DHFR) and an expressible gene encoding a protein of interest. Transfected cells are cultured in the presence of methotrexate (MTX) to produce a plurality of clones. A clone containing plural copy number of the vectors which co-express DHFR and the protein of interest is then selected and cultured The cultured, clone is treated with MTX to enhance the expression of the protein of interest by …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed December 5, 1989 and granted January 19, 1999. A Canadian patent filed from October 1, 1989 lasts 20 years from filing, so its term ended December 5, 2009.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by December 5, 2011.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
Timeline From CIPO dates
Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.