Process for the activation of genetechnologically produced, biologically-active proteins expressed in prokaryotes
CA 2000604 C · Owner: Boehringer Mannheim Gmbh · Inventors: Buchner, Johannes; Rudolph, Rainer; Lenz, Helmut
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
The present invention provides a process for the activation of gene-technologically produced biologically, active proteins expressed in prokaryotes after cell digestion by solubilisation under denaturing conditions and reducing conditions and subsequent reactivation under oxidising and renaturing conditions wherein, working is carried out at a protein concentration of 1 to 1000 µg./ml. and, between the solubilisation and the reactivation, a dialysis is carried out against a buffer with a pH value of from 1 to 4 containing 4 to 8 mole/litre guanidine hydrochloride or 6 to 10 mole/ litre urea.
Read the whole patent
Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Filed October 13, 1989 and granted July 6, 1999. A Canadian patent filed from October 1, 1989 lasts 20 years from filing, so its term ended October 13, 2009.
- A certificate of supplementary protection adds at most two years for a drug, so even that would have ended by October 13, 2011.
- We check Canadian rights only. A patent in its family in another country could still be in force: check its family on Google Patents.
Timeline
Timeline From CIPO dates
Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.