Chemiluminescent acridine derivatives and their use in luminescence immunoassays

Abstract From the Canadian patent

Chemiluminescent acridinium derivative of the formula I (see formula I), in which R1 is hydrogen an alkyl, alkenyl or alkinyl, radical with 1 to 10 carbon atoms or a benzyl or aryl group, R2 and R3 are hydrogen, an alkyl group with 1 to 4 carbon atoms, a substituted or unsubstituted amino group, a carboxyl, C1-C4-alkoxy, cyano or nitro group or halogen, R4 represents a radical in which a sulfonamide group is bonded directly via the nitrogen to the carbonyl group, or is a thioalkyl or thioaryl radical of the formula II, - S - X - R5 (II), in which X is a branched or unbranched aliphatic group or an aromatic group, which can also contain heteroatoms and R5 is a reactive group which can …