Active site modified protease alpha-1-antitrypsin inhibitors and their production

Abstract From the Canadian patent

Proteinaceous compositions are provided which inhibit naturally occurring serine proteases. Particularly an amino acid sequence analogous to human .alpha.1-antitrypsin is modified at the active site while maintaining protease inhibition. The methionine at the active site is substituted with an oxidatively stable amino acid, while other amino acids may also be changed, added or deleted, particularly at the termini. The proteinaceous compositions can be produced in yeast, particularly the S. carlsbergensis/S. cerevisiae hybrid strain AB110 (containing plasmid pC1/1GAPATi9) deposited at the A.T.C.C. on May 9, 1984, and given Accession No. 20709.