Large scale method for purification of high purity heparinase
CA 1341079 C · Owner: Massachusetts Institute Of Technology · Inventors: Zimmerman, Joseph J.; Cooney, Charles L.
Overview
Its claims, description and drawings are in the Canadian Patents Database.
Open the CIPO recordAbstract From the Canadian patent
The present invention is an improved process for purification of active heparinase and heparinase like enzymes from Gram negative organisms, in particular Flavobacterium heparinum. The primary advantage of the process is the fact that it allows large scale processing and high yield of heparinase. The heparinase is released from the periplasmic space of the organism by osmotic shock treatment first into an, osmotically stabilized medium secondly into a , non-stabilized medium having a pH of approximately pH 6.0 and 8.6 with subsequent release into a second non-stabilized medium containing approximately 0.15 M sodium chloride, followed by fractionation by cation exchange chromatography, and, …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Granted August 8, 2000. A Canadian patent filed before October 1, 1989 lasted 17 years from grant, so its term ended August 8, 2017.
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Timeline
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Sources & verification
Official CIPO records
Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.