Purification, cloning and characterization of the gene for interleukin 1
CA 1340978 C · Owner: Larsen, Alf D. · Inventors: Larsen, Alf D.; Price, Virginia L.; Hopp, Thomas P.
Overview
Its claims, description and drawings are in the Canadian Patents Database.
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Interleukin 1 is purified to homogeneity by use of various techniques including ion exchange chromatography and dye-ligand affinity chromatography, thereby enabling the amino acid composition of this protein to be, ascertained and its amino acid sequence to be partially determined. Double-stranded cDNA is prepared from polyadenylated RNA extracted from activated, human peripheral blood adherent mononuclear cells. The cDNA is inserted within a plasmid vector and then the recombinant plasmid employed to transform an appropriate host. Plasmid DNA prepared from pools of transformed hosts is hybridized with a labeled, synthetic oligonucleotide probe corresponding to a portion of the …
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Listed from its CIPO record in Patent Yard's archive of Canadian patents past their term. Its claims, description and drawings are in the CIPO record and on Google Patents, and the Claude kit works from them.
Why it's free to use
- Granted May 2, 2000. A Canadian patent filed before October 1, 1989 lasted 17 years from grant, so its term ended May 2, 2017.
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Everything on this page comes from the CIPO record linked above. Contains information licensed under the Open Government Licence – Canada.