Process for obtaining active proteins from a biologically inactive form

Abstract From the Canadian patent

A process for the preparation of a spatial form which, has biological activity, of a protein from a biologically inactive spatial form is described and comprises the protein being dissolved with the addition of a denaturing agent and thus converted into the random coil form and, the solution being allowed to pass through a material which has molecular sieve properties and contains a liquid medium in which the protein can assume a spatial form which has biological activity, and this material having molecular sieve properties being selected so that the molecules of the denaturing agent can penetrate, but the protein molecules cannot. It is possible by centrifugation blowing or, sucking out to …