Purification and administration of dna repair enzymes

Abstract From the Canadian patent

Methods for purifying DNA repair enzymes are provided in which an aqueous solution of a DNA repair enzyme in an impure state is applied to a molecular sieve column having an exclusion limit which will retard the DNA repair enzyme but will not retard contaminants larger than the DNA repair enzyme. The DNA repair enzyme in an enhanced state of purity is eluted isocratically from the molecular sieve column in an elution buffer and applied directly to a DNA affinity column in the same buffer without intermediate dialysis, ultrafiltration or other procedures. The, DNA repair enzyme is eluted from the DNA affinity column using for example, a salt gradient. The method is rapid inexpensive, simple …