Dna cassettes for making mobilizable cosmids, and vectors carrying these cassettes for use in genetic manipulations of bacteria

Abstract From the Canadian patent

Several embodiments of DNA cassettes and plasmids and cosmids containing portable DNA cassettes are provided herein. One embodiment is a portable DNA cassette into which a mob region of a conjugative, mobilizable, wide-host range plasmid has been cloned, the portable DNA cassette having endonuclease restriction sites at both termini for subsequent cutting by a selected restriction endonuclease. Another embodiment is a plasmid containing a portable DNA cassette into which a mob region of a conjugative, mobilizable, wide-host range plasmid has been cloned, and having endonuclease restriction sites at both termini for subsequent cutting by a selected restriction endonuclease. A third …