Methods for preparing proteins of altered structure

Abstract From the Canadian patent

The present invention relates to methods for inducing cellular mutation by promoting the reaction of nonenzymatic glycosylation of genetic material such as, DNA and RNA, by incubation of the genetic material with certain protein structure modifying agents. Suitable such agents are reducing sugars, such as glucose glucose-6-phosphate and a reactive intermediate comprising a complex between glucose 6-phosphate and lysine. The methods include the in vitro reaction of the agent with genetic material, such as a particular gene-bearing plasmid, under conditions promoting mutation followed by the introduction of the plasmid bearing the reacted gene into a host cell, and allowing the reacted gene …